【ACE C18色譜柱】食用海藻中砷的LC-ICP-MS和LC-ESI-MS分析

Arsenolipids from Edible Seaweed by LC-ICP-MS and LC-ESI-MS

ACE C18, 3μm 150 x 4.6mm
Gradient analysis
A = 0.1% formic acid in H2O
B = 0.1% formic acid in CH3OH
Time (mins) %B
0 0
20 100
45 100
Flow rate: 1ml/min
Injection volume: 100μl
Split ratio: 75% ESI-MS: 25% ICP-MS
Thermo Scientific Element 2 ICP-MS
Mode: Organic mode
Medium resolution
Thermo Scientific Orbitrap Discovery
Positive ESI mode
Spray voltage: 4.5kV
Capillary temperature: 320°C
Capillary voltage: 42V
Separation of arsenic species from methanolic extract of the edible seaweed Alaria esculenta

Arsenic-containing hydrocarbon: 

m/z 389 [M + H]+ for C21H46AsO

Arsenic-containing phospholipids:
m/z 959 [M + H]+ for C45H89AsO14P (C16:0/C16:0)
m/z 987 [M + H]+ for C47H93AsO14P (C18:0/C16:0)
m/z 1015 [M + H]+ for C49H97AsO14P (C20:0/C16:0)