【ACE Excel C18-PFP色譜柱】果汁中維生素的快速液相色譜-質譜分析
Vitamins in Fruit Juice by Fast LC-MS
Conditions
Column: ACE Excel 3 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL -1110-1002U
Mobile Phase: A: 15 mM formic acid, adjusted to pH 3.8 with ammonia solution
B: MeOH
Gradient:
|
Time(mins) |
%B |
|
0.00 |
1 |
|
1.00 |
1 |
|
3.00 |
8 |
|
3.10 |
25 |
|
6.00 |
50 |
|
6.50 |
50 |
|
6.51 |
1 |
|
9.00 |
1 |
Temperature: 30 °C
Detection: LCMS-8040 triple quad MS ESI positive mode (ESI negative for ascorbic and citric acid)
DL temperature: 250 °C
Heat block temperature: 400 °C
Analytes
1. Thiamine (Vitamin B1)(m/z 266.10 122.15)
2. Pyridoxine (Vitamin B6)(m/z 170.20 152.15)
3. Nicotinic acid (Vitamin B3)(m/z 124.00 78.00)
4. Pantothenic acid (Vitamin B5)(m/z 220.30 90.05)
5. Cyanocobalamin (Vitamin B12)(m/z 678.50 147.05)
6. Riboflavin (Vitamin B2)(m/z 377.20 243.10)
7. Biotin (Vitamin B7)(m/z 245.10 227.05)
8. Nicotinamide (Vitamin B3)(m/z 123.20 80.05)
9. Ascorbic acid (Vitamin C)(m/z 175.10 114.80)
10. Citric acid(m/z 191.10 87.15)
