【ACE Excel C18-Amide色譜柱】香蘭素色譜分析

Vanillins

Conditions

Column: ACE Excel 3 C18-Amide
Dimensions: 150 x 4.6 mm
Part Number: EXL -1112-1546U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient:

Time(mins)	%B
0.0	30
10.0	55
10.5	55
15.0	30
20.0	30

Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 40 °C
Detection: UV, 260 nm
Analytes
1. Vanillic acid
2. 4-Hydroxybenzoic acid
3. Vanillin
4. 4-Hydroxybenzaldehyde
5. Guaiacol
6. Ethyl Vanillin
7. Eugenol